lucigen 30243-2 30281-2 NxGen? T4 DNA Ligase RNAse-男女真人后進式猛烈視頻,歐美午夜精品一區(qū)區(qū)電影 Inhibitor

產(chǎn)品簡介：北京中北林格供應lucigen 30243-2 30281-2 NxGen? T4 DNA Ligase RNAse-男女真人后進式猛烈視頻,歐美午夜精品一區(qū)區(qū)電影 Inhibitor，中北林格是lucigen總代理，提供lucigen產(chǎn)品貨號報價查詢。代理的其他品牌包括Biosearch technologies總代理，epicentre總代理，illumina總代理，ICLLAB總代理，ImmunoReagents總代理。</p>

產(chǎn)品名稱：NxGen? T4 DNA Ligase (High Concentration)</p>

貨號：lucigen 30243-2</p>

規(guī)格：7,500 U</p>

存儲溫度：-20o C</p>

 

品牌：lucigen</p>

供應商：中北林格</p>

產(chǎn)地：us</p>

發(fā)貨地：北京</p>

lucigen 30243-2 30281-2 NxGen? T4 DNA Ligase RNAse-男女真人后進式猛烈視頻,歐美午夜精品一區(qū)區(qū)電影 Inhibitor 產(chǎn)品說明書

Reliably join double-stranded nucleic acid chains.
可靠地連接雙鏈核酸鏈。

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

T4 DNA連接酶催化雙鏈DNA或RNA的末端5'磷酸和3'羥基之間的磷酸二酯鍵的形成。該酶有效地連接鈍性和粘性末端并修復雙鏈DNA，RNA或DNA / RNA雜交體中的單鏈缺口。

T4 DNA Ligase VersionSizesLigase ConcentrationProvided BufferLow Concentration Ligase

1,500 U
7,500 U

2 U/μL10X T4 DNA Ligase BufferHigh Concentration Rapid Kit1,500 U
7,500 U10 U/μL2X Rapid Ligation Buffer,
10X T4 DNA Ligase Buffer

T4 DNA連接酶版本尺寸連接酶濃度提供緩沖區(qū)低濃度連接酶

1,500 U 
7,500 U.

2 U /μL10X T4 DNA連接酶緩沖液高濃度快速試劑盒1,500 U 
7,500 U.10 U /μL2X快速連接緩沖液，
10X T4 DNA連接酶緩沖液

10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl2, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.

10X T4 DNA連接酶緩沖液由500mM Tris-HCl，100mM MgCl 2,50mM二硫蘇糖醇，10mM ATP，pH 7.6（25℃）組成。

2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mM MgCl2, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.

2X快速連接緩沖液由132mM Tris-HCl，20mM MgCl 2,2mM二硫蘇糖醇，2mM ATP，15％PEG，pH 7.6（25℃）組成。

Figure 1:  Purity
The purity of the T4 DNA ligase is equal or greater than 95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining.  Molecular weight markers are in Lane 1.  T4 DNA ligase was loaded at 5 units (Lane 2) nd 10 units (Lane 3).
圖1：純度
通過用考馬斯藍染色的SDS-聚丙烯酰胺凝膠電泳判斷，T4 DNA連接酶的純度等于或大于95％。分子量標記在泳道1中.T4 DNA連接酶以5個單位（泳道2）和10個單位（泳道3）加載。

Figure 2:  Exonuclease and Endonuclease Activity Assay
The T4 DNA ligase is free of detectable of exo- and endonuclease activities, as judged by agarose gel electrophoresis following incubation of 10 units of enzyme for 16 hours at 37 °C with 1 μg of HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5 and 6).  Molecular weight markers are in Lanes 1 and 2.

圖2：   核酸外切酶和核酸內(nèi)切酶活性測定
T4 DNA連接酶不含外源和內(nèi)切核酸酶活性，可通過瓊脂糖凝膠電泳判斷，將10單位酶在37℃下與1μgHindⅢ消化后孵育16小時λDNA（泳道3和4）和超螺旋pUC19 DNA（泳道5和6）。分子量標記在泳道1和2中。

Unit Definition: One Weiss Unit is defined as the amount of enzyme required to convert 1 nmol of 32P-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37 °C, using specified reaction conditions. Note: 1 Weiss Unit is approximately 67 cohesive end units.

Source: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.

單位定義： 一個Weiss單位定義為使用特定的反應條件，在37℃下在20分鐘內(nèi)將1nmol 32P標記的無機焦磷酸鹽轉(zhuǎn)化為Norit可吸附材料所需的酶量。注意：1 Weiss Unit約有67個粘性末端單元。

來源：   攜帶克隆的T4 DNA連接酶基因的重組  大腸桿菌菌株。

Contact your local distributor for pricingProduct DescriptionSizeCat. No.NxGen? T4 DNA Ligase (Low Concentration)
1,500 U30241-1
7,500 U30241-2NxGen? T4 DNA Ligase (High Concentration)
1,500 U30243-1
7,500 U30243-2產(chǎn)品描述尺寸貨號NxGen?T4DNA連接酶（低濃度）
1,500 U.30241-1
7,500 U.30241-2NxGen?T4DNA連接酶（高濃度）
1,500 U.30243-1
7,500 U.30243-2ORDER INFORMATION

Storage buffer: T4 DNA Ligase is supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 0.1%Triton X-100, 50% glycerol, pH 7.5 @ 25 °C.

訂單信息

儲存緩沖液：  T4 DNA連接酶以10mM Tris-HCl，50mM KCl，1mM二硫蘇糖醇，0.1mM EDTA，0.1％Triton X-100,50％甘油，pH7.5在25℃下提供。

對此產(chǎn)品有興趣，可聯(lián)系中北林格獲取更多詳細信息。</p>